To make the technique work in cultured mammalian cells, researchers must deliver small interfering RNAs (siRNAs), which are dsRNAs of 21-25 nucleotides, to the cell. They can use a wide range of ...
Problem: Transfecting any siRNA may lead to off-target changes in gene expression. Controls: Do at least one negative control that doesn't target any endogenous gene, says Ruiz-Vela. He generally uses ...
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